Fluorescence In Situ Hybridization

The ALL FISH Panel uses probes to detect several translocations seen in B lymphoblastic leukemia/lymphoma with recurrent genetic abnormalities: BCR/ ABL1, MLL, and ETV6(TEL)/RUNX1(AML1). The ETV6/RUNX1 translocation is often undetectable by routine cytogenetics, making FISH a particularly important tool to exclude this abnormality.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or
purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.

Store and transport at room temperature.

88377

Global

48-72 hours

This test is used to aid in the diagnosis of acute promyelocytic leukemia with PML-RARA. This probe is also available as part of the AML FISH Panel.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.

Store and transport at room temperature.

88377

Global

Performed as STAT with preliminary results available 24 Hours from receipt in lab.

The AML FISH Panel uses probes to detect several abnormalities seen in AML with recurrent genetic abnormalities: RUNX1T1/RUNX1, PML/RARA, CBFB, and MLL. Abnormalities of CBFB and MLL are often undetectable by routine cytogenetics, making FISH a particularly important tool for detection.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.

Store and transport at room temperature.

88377

Global

48-72 hours

This test is used to aid in the diagnosis of acute myeloid leukemia with inv(16) (p13.1q22) or t(16;16)(p13.1;q22);CBFB-MYH11. This probe is also available as part of the AML FISH Panel.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.

Store and transport at room temperature.

88377

Global

48-72 Hours 

This ALK FISH analysis is used to detect ALK rearrangements in non-small cell lung cancer (NSCLC) to aid in identifying patients eligible for treatment with Xalkori® (crizotinib). See ALK (2p23) FISH for ALK testing recommended in anaplastic large cell lymphoma.

Fresh tissue: 1 cm³ tissue completely immersed in RPMI.
Paraffin block: FFPE block label with patient name & ID number and 1 H&E
stained slide with tumor encircled.
Unstained slides: 3 unstained charged slides and 1 H&E slide cut at 4 microns. Clearly label with patient name and ID number.

UroVysion Bladder Cancer FISH Analysis is designed to detect aneuploidy for chromosomes 3, 7, 17, and/or loss of the 9p21 locus in urine specimens from patients suspected of having bladder cancer. Results from the UroVysion test are intended for use in conjunction with, but not in lieu of, current standard diagnostic procedures for initial diagnosis of bladder carcinoma in patients with hematuria and for monitoring for tumor recurrence in patients previously diagnosed with bladder cancer.

Less than 33 ml voided urine, fresh or in PreservCyt vial 

Refrigerate until transport. Frozen unacceptable. 

88377

Global

Tech Only

48-72 Hours

The ALK Dual Color, Break Apart Rearrangement Probe is designed to detect the known ALK (2p23) rearrangements that occur in anaplastic large cell lymphoma including t(2;5) and its variants.
See “ALK FISH for NSCLC” for ALK testing recommended in non-small cell lung cancer.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.
>Fresh tissue: 1 cm³ tissue completely immersed in RPMI.
Paraffin block: FFPE block label with patient name & ID number and 1 H&E stained slide with tumor encircled.
Unstained slides: 3 unstained charged slides and 1 H&E slide cut at 4 microns. Clearly label with patient name and ID number.

BM/PB, block or unstained slides: Store and transport at room temperature.
Fresh tissue: Refrigerate until transport; ship with cool pack.

88377

Global

48-72 Hours

The MYC Dual Color Break Apart Rearrangement Probe is intended to detect chromosomal rearrangements involving the MYC gene on chromosome 8q24. Translocations involving MYC have diagnostic and prognostic importance in B-cell lymphomas including Burkitt lymphoma and high-grade B-cell lymphoma, with MYC and BCL2 and/or BCL6 rearrangements (double/triple-hit lymphomas). This probe is also available as part of the Non-Hodgkin Lymphoma and Large B-Cell Lymphoma Panels.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.
Fresh tissue: 1 cm³ tissue completely immersed in RPMI.
Paraffin block: FFPE block labeled with patient name & ID number and 1 H&E stained slide with tumor encircled. Unstained slides: 3 unstained charged slides and 1 H&E Slide cut at 4 microns. Clearly label with patient name and ID number.

BM/PB, block or unstained slides: Store and transport at room temperature.
Fresh tissue: Refrigerate until transport; ship with cool pack.

88377

Global

48-72 Hours

This test is used to aid in the diagnosis of acute myeloid leukemia with t(8;21 (q22;q22.1); RUNX1-RUNX1T1. This probe is also available as part of the AML FISH Panel.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.

Store and transport at room temperature.

88377

Global

48-72 Hours 

This FISH Panel includes probes to detect various recurrent abnormalities seen in chronic lymphocytic leukemia (CLL) and the CCND1/IGH rearrangement seen in the vast majority of mantle cell lymphomas. This panel is useful to help establish an initial diagnosis of CLL and exclude mantle cell lymphoma and to assess important prognostic markers in CLL.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.

Store and transport at room temperature.

88377, 88367

Global

48-72 Hours

The IGH/BCL2 Dual Color, Dual Fusion Translocation Probe is designed to detect the translocation involving IGH at 14q32 and BCL2 at 18q21, t(14;18)(q32;q21).
This rearrangement is found in the majority of follicular lymphomas and in a subset of diffuse large B-cell lymphomas and high-grade B-cell lymphomas, with MYC and BCL2 and/or BCL6 rearrangements (double/triple-hit lymphomas). This probe is also available as part of the Non-Hodgkin Lymphoma and Large B-Cell Lymphoma Panels.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.
Fresh tissue: 1 cm³ tissue completely immersed in RPMI.
Paraffin block: FFPE block labeled with patient name & ID number and 1 H&E
stained slide with tumor encircled.
Unstained slides: 3 unstained charged slides and 1 H&E Slide cut at 4 microns. Clearly label with patient name and ID number.

BM/PB, block or unstained slides: Store and transport at room temperature.
Fresh tissue: Refrigerate until transport; ship with cool pack.

88377

Global

48-72 Hours

This panel can detect several common translocations seen in large B-cell lymphomas and is useful to evaluate for high-grade B-cell lymphoma, with MYC and BCL2 and/or BCL6 rearrangements (double/triple-hit lymphomas),

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.
Fresh tissue: 1 cm³ tissue completely immersed in RPMI.
Paraffin block: FFPE block labeled with patient name & ID number and 1 H&E
stained slide with tumor encircled.
Unstained slides: 3 unstained charged slides and 1 H&E Slide cut at 4 microns Clearly label with patient name and ID number.

BM/PB, block or unstained slides: Store and transport at room temperature.
Fresh tissue: Refrigerate until transport; ship with cool pack.

88377

Global

48-72 Hours

HER2 testing in breast cancer is used to assess prognosis and eligibility for trastuzumab (Herceptin®) treatment. In gastric cases, it is also used to determine eligibility for anti-HER2 treatments.

Fresh tissue: 1 cm³ tissue completely immersed in RPMI.
Paraffin block: FFPE block labeled with patient name & ID number and 1 H&E
stained slide with tumor encircled.
Unstained slides: 3 unstained charged slides and 1 H&E Slide cut at 4 microns. Clearly label with patient name and ID number.

Block or unstained slides: Store and transport at room temperature.
Fresh tissue: Refrigerate until transport; ship with cool pack.

88377

Global

48-72 Hours

This panel is used to detect copy number gains of the RREB1 (6p region) and CCND1 (11q region) genes as well as copy number loss of the MYB (6q region). Detection of these abnormalities can help distinguish melanomas from nevi.

Fresh tissue: 1 cm³ tissue completely immersed in RPMI.
Paraffin block: FFPE block labeled with patient name & ID number and 1 H&E
stained slide with tumor encircled.
Unstained slides: 3 unstained charged slides and 1 H&E Slide cut at 4 microns. Clearly label with patient name and ID number.

BM/PB, block or unstained slides: Store and transport at room temperature.
Fresh tissue: Refrigerate until transport; ship with cool pack.

88377

Global

48-72 Hours

The IGH/MALT1 Dual Color, Dual Fusion Translocation Probe is designed to detect the translocation involving IGH at 14q32 and MALT1 at 18q21, t(14;18) (q32;q21). This translocation is seen in a subset of extranodal marginal zone lymphomas of mucosa-associated lymphoid tissue (MALT lymphomas). This probe is also available as part of the Non-Hodgkin Lymphoma Panel.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube. Fresh tissue: 1 cm³ tissue completely immersed in RPMI.
Paraffin block: FFPE block labeled with patient name & ID number and 1 H&E
stained slide with tumor encircled.
Unstained slides: 3 unstained charged slides and 1 H&E Slide cut at 4 microns. Clearly label with patient name and ID number.

BM/PB, block or unstained slides: Store and transport at room temperature.
Fresh tissue: Refrigerate until transport; ship with cool pack.

88377

Global

48-72 Hours

This panel is used to detect common abnormalities seen in multiple myeloma (plasma cell myeloma), which are often undetectable by routine cytogenetics. Detection of one or more of these abnormalities can help to establish a diagnosis of myeloma and provide prognostic information. The Multiple Myeloma Reflex Panel is recommended when IGH rearrangement is detected to determine the specific fusion partner and prognostic implications.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.

Store and transport at room temperature.

88377

Global

48-72 Hours

The CCND1(BCL1)/IGH Dual Color, Dual Fusion Translocation Probe is designed to detect the translocation involving CCND1(BCL1) at 11q13 and IGH at 14q32, t(11;14)(q13;q32).
This translocation is seen in the vast majority of mantle cell lymphomas but is generally not seen in other B-cell lymphomas. This translocation is also seen in a subset of plasma cell myelomas. This probe is also available as part of the CLL and Non-Hodgkin Lymphoma Panels.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.
Fresh tissue: 1 cm³ tissue completely immersed in RPMI.
Paraffin block: FFPE block labeled with patient name & ID number and 1 H&E
stained slide with tumor encircled.
Unstained slides: 3 unstained charged slides and 1 H&E Slide cut at 4 microns. Clearly label with patient name and ID number.

BM/PB, block or unstained slides: Store and transport at room temperature.
Fresh tissue: Refrigerate until transport; ship with cool pack.

88377

Global

48-72 Hours

This panel is mainly used to help determine the specific fusion partner and prognostic implications when an IGH rearrangement is detected in the Multiple Myeloma FISH Panel, although the panel can also be ordered as a stand-alone test if clinically indicated.
The common IGH fusion partners that can be detected include CCND1 (11q13), FGFR3 (4p16.3), and C-MAF (16q23).

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.

88377

Global

48-72 Hours

The BCL6 Dual Color, Break Apart Rearrangement Probe is used to detect translocations involving the BCL6 gene. BCL6 translocations are seen in diffuse large B-cell lymphoma; high-grade B-cell lymphoma, with MYC and BCL2 and/or BCL6 rearrangements; and other B-cell lymphoproliferative disorders. This probe is also available as part of the Non-Hodgkin Lymphoma and Large B-Cell Lymphoma Panels.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or  purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.
Fresh tissue: 1 cm³ tissue completely immersed in RPMI.
Paraffin block: FFPE block labeled with patient name & ID number and 1 H&E stained slide with tumor encircled. Unstained slides: 3 unstained charged slides and 1 H&E Slide cut at 4 microns. Clearly label with patient name and ID number.

BM/PB, block or unstained slides: Store and transport at room temperature.
Fresh tissue: Refrigerate until transport; ship with cool pack.

88377

Global

48-72 Hours

The Non-Hodgkin Lymphoma FISH Panel includes probes to detect a range of recurrent genetic abnormalities seen in non-Hodgkin lymphoma that are useful for diagnosis and subclassification.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.
Fresh tissue: 1 cm³ tissue completely immersed in RPMI.
Paraffin block: FFPE block labeled with patient name & ID number and 1 H&E
stained slide with tumor encircled.
Unstained slides: Min14 unstained charged slides and 1 H&E Slide cut at 4 microns. Clearly label with patient name and ID number.

BM/PB, block or unstained slides: Store and transport at room temperature.
Fresh tissue: Refrigerate until transport; ship with cool pack.

88377

Global

48-72 Hours

This analysis is used to detect PTEN deletion and TMPRSS2:ERG rearrangement, which have been associated with adverse prognosis in prostate cancer..

Fresh tissue: 1 cm³ tissue completely immersed in RPMI.
Paraffin block: FFPE block labeled with patient name & ID number and 1 H&E stained slide with tumor encircled. Unstained slides: Minimum 4 unstained charged slides and 1 H&E Slide cut at 4 microns. Clearly label with patient name and ID number.

Block or unstained slides: Store and transport at room temperature.
Fresh tissue: Refrigerate until transport; ship with cool pack.

88377

Global

48-72 Hours

This panel is used to detect common abnormalities seen in myelodysplastic syndromes (MDS) and other myeloid neoplasms, including gain of chromosome 8, deletion of chromosome 7/7q, deletion of chromosome 5/5q, deletion of chromosome 20q, and deletion of chromosome 11q. Detection of one or more of these abnormalities can help to establish a diagnosis of MDS and provide prognostic information.

Bone marrow aspirate: Minimum 2 ml in green top (sodium heparin) tube or purple top (EDTA) tube.
Peripheral blood: Minimum 5 ml in green top (sodium heparin) or purple top (EDTA) tube.

Store and transport at room temperature.

88377

Global

48-72 Hours